Note: If we have multiple samples in one well, we only process the first one (alphanumerical order). Important: The number of lines contained in the MassLynx template file will be cropped to fit the current number of samples that was process in this samples prep run. Example of data file are in the attachment. Twenty-four hours after transfection, lipids were extracted, and ceramide species were analyzed by a Xevo TQ-S LC/MS system and quantified by MassLynx software. HEK 293T cells were transfected with a vector or a plasmid encoding WT HA-CERS16 or MT HA-CERS26, as indicated.
#MASSLYNX SOFTWARE SEETINGS SOFTWARE#
Learn more about the sample info export data, see Annex: Complete and retrieve ?️ sample info at the end of the experiment At the workplace, we use MassLynx, but I need to work from home with these data, and I need a free software to view and process chromatograms. Figure Legend Snippet: Phosphorylation is required for CERS26 to function in cells. We add the sample position on 2 digits after the colon (well numbering of the plate in sequential discontinuous format (column-wise numbering from 1-96), like SamplePositionNumbering in the other samples info export file) O The Bed position (before the colon), being the location the extracted samples in the 96 well plate are placed into the sample manager/organizer of the LC/MS system, is retrieved from the MassLynx template file If the LabwareIdentifier of the source was not provided by the operator, OneLab will use the OneLab UUID of the parent labware ( SampleParentOneLabUUID) Otherwise, we use the LabwareIdentifier of the source and we concatenate with the SamplePositionNumbering by following this format: LabwareParentIdentifier_SamplePositionNumbering
#MASSLYNX SOFTWARE SEETINGS CODE#
If available, we use the SampleIdentifier of the source ( SampleParentIdentifier) or sample bar code scanned in using a USB hand held scanner. The Sample ID columns is filled with the sample information available in OneLab following these rules: This exported file contains all the settings contained in the MassLynx template file plus all the gaps filled by OneLab:Ī unique File Name is generated with date and time as a prefix and an increment on 3 digits as a suffix ? Now, you can start the measurements with your instrument!Īnnex: What OneLab does with the MassLynx template file. Once the MassLynx sample list has been created, it should be saved and the sample list can then be submitted.